Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2067646..2068300 Replicon chromosome
Accession NZ_CP114893
Organism Escherichia coli strain CM13

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag O6R07_RS10130 Protein ID WP_000244777.1
Coordinates 2067893..2068300 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag O6R07_RS10125 Protein ID WP_000354046.1
Coordinates 2067646..2067912 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6R07_RS10100 (2062815) 2062815..2063558 + 744 WP_000951964.1 SDR family oxidoreductase -
O6R07_RS10105 (2063615) 2063615..2065048 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
O6R07_RS10110 (2065093) 2065093..2065404 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
O6R07_RS10115 (2065568) 2065568..2066227 + 660 WP_000250274.1 hemolysin III family protein -
O6R07_RS10120 (2066423) 2066423..2067403 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
O6R07_RS10125 (2067646) 2067646..2067912 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
O6R07_RS10130 (2067893) 2067893..2068300 + 408 WP_000244777.1 protein YgfX Toxin
O6R07_RS10135 (2068340) 2068340..2068861 - 522 WP_001055874.1 flavodoxin FldB -
O6R07_RS10140 (2068973) 2068973..2069869 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
O6R07_RS10145 (2069894) 2069894..2070604 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
O6R07_RS10150 (2070610) 2070610..2072343 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T266951 WP_000244777.1 NZ_CP114893:2067893-2068300 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT266951 WP_000354046.1 NZ_CP114893:2067646-2067912 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References