266942

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 275813..276492 Replicon chromosome
Accession NZ_CP114893
Organism Escherichia coli strain CM13

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag O6R07_RS01435 Protein ID WP_000854672.1
Coordinates 276151..276492 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag O6R07_RS01430 Protein ID WP_000070395.1
Coordinates 275813..276130 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6R07_RS01380 (270860) 270860..271723 + 864 WP_001065553.1 GTPase family protein -
O6R07_RS01385 (271815) 271815..272636 + 822 WP_000197389.1 DUF932 domain-containing protein -
O6R07_RS01390 (272853) 272853..273044 + 192 Protein_275 DeoR family transcriptional regulator -
O6R07_RS01395 (273040) 273040..273267 + 228 WP_001548158.1 protein YpjK -
O6R07_RS01400 (273267) 273267..273710 + 444 WP_269707801.1 lipoprotein YafY -
O6R07_RS01405 (273733) 273733..274200 + 468 WP_001547765.1 protein YkfB -
O6R07_RS01410 (274277) 274277..274516 + 240 WP_000194654.1 DUF905 family protein -
O6R07_RS01415 (274614) 274614..275072 + 459 WP_000211838.1 antirestriction protein -
O6R07_RS01420 (275088) 275088..275564 + 477 WP_000811693.1 RadC family protein -
O6R07_RS01425 (275573) 275573..275794 + 222 WP_000691994.1 DUF987 domain-containing protein -
O6R07_RS01430 (275813) 275813..276130 + 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
O6R07_RS01435 (276151) 276151..276492 + 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
O6R07_RS01445 (277064) 277064..278317 - 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
O6R07_RS01450 (278329) 278329..279432 - 1104 WP_001285288.1 glutamate 5-kinase -
O6R07_RS01455 (279720) 279720..280775 + 1056 WP_000749863.1 phosphoporin PhoE -
O6R07_RS01460 (280814) 280814..281215 - 402 WP_000174689.1 sigma factor-binding protein Crl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T266942 WP_000854672.1 NZ_CP114893:276151-276492 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT266942 WP_000070395.1 NZ_CP114893:275813-276130 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References