Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3585190..3585687 Replicon chromosome
Accession NZ_CP114892
Organism Escherichia coli strain CM5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag O6Q95_RS17470 Protein ID WP_001173343.1
Coordinates 3585514..3585687 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag O6Q95_RS17465 Protein ID WP_000398619.1
Coordinates 3585190..3585486 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6Q95_RS17445 (3581921) 3581921..3582640 - 720 WP_000611288.1 two-component system response regulator DcuR -
O6Q95_RS17450 (3582637) 3582637..3584268 - 1632 WP_001216477.1 sensor histidine kinase -
O6Q95_RS17455 (3584449) 3584449..3584679 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
O6Q95_RS17460 (3584691) 3584691..3584963 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
O6Q95_RS17465 (3585190) 3585190..3585486 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
O6Q95_RS17470 (3585514) 3585514..3585687 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
O6Q95_RS17475 (3585806) 3585806..3587323 - 1518 WP_001295074.1 lysine--tRNA ligase -
O6Q95_RS17480 (3587560) 3587560..3589017 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T266934 WP_001173343.1 NZ_CP114892:3585514-3585687 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT266934 WP_000398619.1 NZ_CP114892:3585190-3585486 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References