Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 319863..320517 Replicon chromosome
Accession NZ_CP114891
Organism Escherichia coli strain CM2

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag O6Q44_RS01620 Protein ID WP_000244777.1
Coordinates 320110..320517 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag O6Q44_RS01615 Protein ID WP_000354046.1
Coordinates 319863..320129 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6Q44_RS01590 (315032) 315032..315775 + 744 WP_000951964.1 SDR family oxidoreductase -
O6Q44_RS01595 (315832) 315832..317265 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
O6Q44_RS01600 (317310) 317310..317621 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
O6Q44_RS01605 (317785) 317785..318444 + 660 WP_000250274.1 hemolysin III family protein -
O6Q44_RS01610 (318640) 318640..319620 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
O6Q44_RS01615 (319863) 319863..320129 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
O6Q44_RS01620 (320110) 320110..320517 + 408 WP_000244777.1 protein YgfX Toxin
O6Q44_RS01625 (320557) 320557..321078 - 522 WP_001055874.1 flavodoxin FldB -
O6Q44_RS01630 (321190) 321190..322086 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
O6Q44_RS01635 (322111) 322111..322821 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
O6Q44_RS01640 (322827) 322827..324560 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T266895 WP_000244777.1 NZ_CP114891:320110-320517 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT266895 WP_000354046.1 NZ_CP114891:319863-320129 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References