Detailed information of TA system TA026689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2050674..2051203
• Replicon: chromosome
• Accession: NC_017343
• Organism: Staphylococcus aureus subsp. aureus ECT-R 2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ECTR2_RS10260
• Protein ID: WP_000621175.1
• Coordinates: 2050674..2051036 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ECTR2_RS10265
• Protein ID: WP_000948331.1
• Coordinates: 2051033..2051203 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTR2_RS10235	2047652..2048422	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
ECTR2_RS10240	2048397..2048876	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
ECTR2_RS10245	2048878..2049204	-	327	WP_001052491.1	anti-sigma factor antagonist	-
ECTR2_RS10250	2049323..2050324	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ECTR2_RS10260	2050674..2051036	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ECTR2_RS10265	2051033..2051203	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ECTR2_RS10270	2051288..2052436	-	1149	WP_001281154.1	alanine racemase	-
ECTR2_RS10275	2052502..2052861	-	360	WP_000581197.1	holo-ACP synthase	-
ECTR2_RS10280	2052865..2053356	-	492	WP_001205912.1	PH domain-containing protein	-
ECTR2_RS10285	2053343..2054926	-	1584	WP_001294626.1	PH domain-containing protein	-
ECTR2_RS10290	2054919..2055398	-	480	WP_001287087.1	hypothetical protein	-
ECTR2_RS10295	2055606..2056166	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T26689 WP_000621175.1 NC_017343:c2051036-2050674 [Staphylococcus aureus subsp. aureus ECT-R 2]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T26689 NC_017343:c2051036-2050674 [Staphylococcus aureus subsp. aureus ECT-R 2]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT26689 WP_000948331.1 NC_017343:c2051203-2051033 [Staphylococcus aureus subsp. aureus ECT-R 2]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT26689 NC_017343:c2051203-2051033 [Staphylococcus aureus subsp. aureus ECT-R 2]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1