Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4646887..4647697 Replicon chromosome
Accession NZ_CP114853
Organism Klebsiella pneumoniae strain S245

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag O5O52_RS22635 Protein ID WP_004178461.1
Coordinates 4646887..4647420 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag O5O52_RS22640 Protein ID WP_002887278.1
Coordinates 4647431..4647697 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O5O52_RS22630 (4645718) 4645718..4646839 + 1122 WP_004186850.1 cupin domain-containing protein -
O5O52_RS22635 (4646887) 4646887..4647420 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
O5O52_RS22640 (4647431) 4647431..4647697 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
O5O52_RS22645 (4647800) 4647800..4649233 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
O5O52_RS22650 (4649223) 4649223..4649906 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
O5O52_RS22655 (4650079) 4650079..4651464 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
O5O52_RS22660 (4651482) 4651482..4651826 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T266867 WP_004178461.1 NZ_CP114853:c4647420-4646887 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT266867 WP_002887278.1 NZ_CP114853:c4647697-4647431 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References