Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 16484..17592 Replicon plasmid pEF0656-3
Accession NZ_CP114620
Organism Enterococcus faecium strain EF0656

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag O4N81_RS14975 Protein ID WP_000233000.1
Coordinates 16723..17592 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag O4N81_RS14970 Protein ID WP_000205227.1
Coordinates 16484..16708 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O4N81_RS14950 (O4N81_14950) 12870..13550 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
O4N81_RS14955 (O4N81_14955) 13863..14360 + 498 WP_002327635.1 DNA recombinase -
O4N81_RS14960 (O4N81_14960) 14361..14777 + 417 WP_000323438.1 recombinase -
O4N81_RS14965 (O4N81_14965) 14779..16341 + 1563 WP_000136908.1 recombinase family protein -
O4N81_RS14970 (O4N81_14970) 16484..16708 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
O4N81_RS14975 (O4N81_14975) 16723..17592 + 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
O4N81_RS14980 (O4N81_14980) 17573..18307 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
O4N81_RS14985 (O4N81_14985) 18340..18603 + 264 Protein_20 aminoglycoside 6-adenylyltransferase -
O4N81_RS14990 (O4N81_14990) 18658..19338 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
O4N81_RS14995 (O4N81_14995) 19431..20225 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
O4N81_RS15000 (O4N81_15000) 20318..20860 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
O4N81_RS15005 (O4N81_15005) 20857..21534 - 678 Protein_24 aminoglycoside 6-adenylyltransferase -
O4N81_RS15010 (O4N81_15010) 21564..22244 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T266796 WP_000233000.1 NZ_CP114620:16723-17592 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT266796 WP_000205227.1 NZ_CP114620:16484-16708 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References