Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 35281..36418 Replicon plasmid pEF0656-2
Accession NZ_CP114619
Organism Enterococcus faecium strain EF0656

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag O4N81_RS14835 Protein ID WP_182482948.1
Coordinates 35555..36418 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag O4N81_RS14830 Protein ID WP_000301765.1
Coordinates 35281..35553 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O4N81_RS14800 (O4N81_14800) 30285..31724 + 1440 WP_094899393.1 aminoglycoside O-phosphotransferase APH(2'')-Ia -
O4N81_RS14805 (O4N81_14805) 31854..33026 - 1173 WP_000195429.1 IS256-like element IS256 family transposase -
O4N81_RS14810 (O4N81_14810) 33129..33527 + 399 Protein_35 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
O4N81_RS14815 (O4N81_14815) 33697..33957 + 261 Protein_36 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
O4N81_RS14820 (O4N81_14820) 34060..34956 + 897 WP_118215883.1 ParA family protein -
O4N81_RS14825 (O4N81_14825) 35048..35263 + 216 WP_001835296.1 peptide-binding protein -
O4N81_RS14830 (O4N81_14830) 35281..35553 + 273 WP_000301765.1 antitoxin Antitoxin
O4N81_RS14835 (O4N81_14835) 35555..36418 + 864 WP_182482948.1 zeta toxin family protein Toxin
O4N81_RS14840 (O4N81_14840) 36855..37172 + 318 WP_002300567.1 hypothetical protein -
O4N81_RS14845 (O4N81_14845) 37211..37708 + 498 WP_169040278.1 molecular chaperone DnaJ -
O4N81_RS14850 (O4N81_14850) 37798..37989 - 192 Protein_43 ISL3 family transposase -
O4N81_RS14855 (O4N81_14855) 38356..38934 + 579 WP_032490934.1 TetR/AcrR family transcriptional regulator -
O4N81_RS14860 (O4N81_14860) 38966..39409 + 444 WP_032490933.1 small multi-drug export protein -
O4N81_RS14865 (O4N81_14865) 39562..39747 + 186 WP_169040279.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / aac(6')-aph(2'') - 1..45954 45954


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32432.98 Da        Isoelectric Point: 6.6610

>T266795 WP_182482948.1 NZ_CP114619:35555-36418 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISAKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPIPPIPKTPKLPGL

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT266795 WP_000301765.1 NZ_CP114619:35281-35553 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References