Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4298800..4299297 Replicon chromosome
Accession NZ_CP114592
Organism Escherichia coli strain WEC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag O3296_RS20730 Protein ID WP_001173343.1
Coordinates 4298800..4298973 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag O3296_RS20735 Protein ID WP_000398619.1
Coordinates 4299001..4299297 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3296_RS20720 (4295470) 4295470..4296927 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
O3296_RS20725 (4297164) 4297164..4298681 + 1518 WP_001295074.1 lysine--tRNA ligase -
O3296_RS20730 (4298800) 4298800..4298973 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
O3296_RS20735 (4299001) 4299001..4299297 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
O3296_RS20740 (4299524) 4299524..4299796 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
O3296_RS20745 (4299808) 4299808..4300038 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
O3296_RS20750 (4300219) 4300219..4301850 + 1632 WP_001216487.1 sensor histidine kinase -
O3296_RS20755 (4301847) 4301847..4302566 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T266775 WP_001173343.1 NZ_CP114592:c4298973-4298800 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT266775 WP_000398619.1 NZ_CP114592:c4299297-4299001 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References