Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3553590..3554427 Replicon chromosome
Accession NZ_CP114592
Organism Escherichia coli strain WEC

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag O3296_RS17320 Protein ID WP_000227784.1
Coordinates 3553885..3554427 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag O3296_RS17315 Protein ID WP_001297137.1
Coordinates 3553590..3553901 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3296_RS17290 (3548610) 3548610..3549557 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
O3296_RS17295 (3549579) 3549579..3551570 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
O3296_RS17300 (3551560) 3551560..3552174 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
O3296_RS17305 (3552174) 3552174..3552503 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
O3296_RS17310 (3552515) 3552515..3553405 + 891 WP_000971336.1 heme o synthase -
O3296_RS17315 (3553590) 3553590..3553901 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
O3296_RS17320 (3553885) 3553885..3554427 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
O3296_RS17325 (3554483) 3554483..3555418 - 936 WP_001297127.1 tetratricopeptide repeat protein -
O3296_RS17330 (3555826) 3555826..3557190 + 1365 WP_001000974.1 MFS transporter -
O3296_RS17335 (3557318) 3557318..3557809 - 492 WP_001138904.1 nucleotide binding protein YajQ -
O3296_RS17340 (3557977) 3557977..3558888 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T266771 WP_000227784.1 NZ_CP114592:3553885-3554427 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT266771 WP_001297137.1 NZ_CP114592:3553590-3553901 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References