Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 95450..97073 Replicon plasmid pE1532-MCR
Accession NZ_CP114574
Organism Enterobacter hormaechei strain E1532

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag O2601_RS24760 Protein ID WP_000088044.1
Coordinates 95748..97073 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag O2601_RS24755 Protein ID WP_012477377.1
Coordinates 95450..95743 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O2601_RS24735 (O2601_24735) 91249..91641 + 393 WP_000341066.1 hypothetical protein -
O2601_RS24740 (O2601_24740) 92300..92956 + 657 WP_000462754.1 trypsin-like serine protease -
O2601_RS24745 (O2601_24745) 93159..93656 + 498 WP_000340139.1 membrane protein -
O2601_RS24750 (O2601_24750) 93661..95049 + 1389 WP_012695445.1 hypothetical protein -
O2601_RS24755 (O2601_24755) 95450..95743 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
O2601_RS24760 (O2601_24760) 95748..97073 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
O2601_RS24765 (O2601_24765) 97134..97340 + 207 WP_000134171.1 hypothetical protein -
O2601_RS24770 (O2601_24770) 97441..97851 + 411 WP_000985909.1 hypothetical protein -
O2601_RS24775 (O2601_24775) 97864..98388 + 525 WP_001805097.1 hypothetical protein -
O2601_RS24780 (O2601_24780) 98570..99184 - 615 Protein_94 IS110-like element IS4321 family transposase -
O2601_RS24785 (O2601_24785) 99240..100208 - 969 WP_072202995.1 IS5 family transposase -
O2601_RS24790 (O2601_24790) 100241..100639 - 399 Protein_96 IS110 family transposase -
O2601_RS24795 (O2601_24795) 100730..101164 - 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / qnrB4 / blaDHA-1 / sul1 / blaSHV-12 / aac(3)-IId / mph(A) / aph(3')-Ia / mcr-9 / aph(6)-Id / aph(3'')-Ib - 1..308217 308217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T266734 WP_000088044.1 NZ_CP114574:95748-97073 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT266734 WP_012477377.1 NZ_CP114574:95450-95743 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References