Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35250..35893 Replicon plasmid pE1532-KPC
Accession NZ_CP114573
Organism Enterobacter hormaechei strain E1532

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag O2601_RS24275 Protein ID WP_000754566.1
Coordinates 35250..35666 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag O2601_RS24280 Protein ID WP_001261276.1
Coordinates 35663..35893 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O2601_RS24255 (O2601_24255) 32081..32626 + 546 WP_001493763.1 plasmid pRiA4b ORF-3 family protein -
O2601_RS24260 (O2601_24260) 32763..33335 + 573 WP_001493762.1 recombinase family protein -
O2601_RS24265 (O2601_24265) 33372..34763 + 1392 WP_014839878.1 ISKra4-like element ISKpn19 family transposase -
O2601_RS24270 (O2601_24270) 34795..35046 - 252 Protein_33 transposase -
O2601_RS24275 (O2601_24275) 35250..35666 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
O2601_RS24280 (O2601_24280) 35663..35893 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
O2601_RS24285 (O2601_24285) 36467..36817 + 351 WP_000493378.1 hypothetical protein -
O2601_RS24290 (O2601_24290) 36868..37611 + 744 WP_000129823.1 hypothetical protein -
O2601_RS24295 (O2601_24295) 37608..38384 + 777 WP_000015958.1 site-specific integrase -
O2601_RS24300 (O2601_24300) 38442..38699 - 258 WP_000764642.1 hypothetical protein -
O2601_RS24305 (O2601_24305) 38828..38932 - 105 WP_032409716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / blaKPC-2 - 1..39461 39461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T266733 WP_000754566.1 NZ_CP114573:c35666-35250 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT266733 WP_001261276.1 NZ_CP114573:c35893-35663 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References