Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4700554..4701364 Replicon chromosome
Accession NZ_CP114375
Organism Klebsiella pneumoniae strain KP6870155

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NOZ09_RS23310 Protein ID WP_004178461.1
Coordinates 4700554..4701087 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NOZ09_RS23315 Protein ID WP_002887278.1
Coordinates 4701098..4701364 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NOZ09_RS23305 (4699385) 4699385..4700506 + 1122 WP_040188022.1 cupin domain-containing protein -
NOZ09_RS23310 (4700554) 4700554..4701087 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NOZ09_RS23315 (4701098) 4701098..4701364 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NOZ09_RS23320 (4701467) 4701467..4702900 - 1434 WP_065782632.1 Cu(+)/Ag(+) sensor histidine kinase -
NOZ09_RS23325 (4702890) 4702890..4703573 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NOZ09_RS23330 (4703746) 4703746..4705131 + 1386 WP_065782630.1 efflux transporter outer membrane subunit -
NOZ09_RS23335 (4705149) 4705149..4705493 + 345 WP_065519257.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T266650 WP_004178461.1 NZ_CP114375:c4701087-4700554 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT266650 WP_002887278.1 NZ_CP114375:c4701364-4701098 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References