Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5490935..5491560 Replicon chromosome
Accession NZ_CP114361
Organism Klebsiella variicola strain 2022CK-00568

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag OPU52_RS26560 Protein ID WP_008807903.1
Coordinates 5490935..5491318 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OPU52_RS26565 Protein ID WP_004150355.1
Coordinates 5491318..5491560 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPU52_RS26545 (OPU52_26540) 5488301..5489203 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OPU52_RS26550 (OPU52_26545) 5489200..5489835 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
OPU52_RS26555 (OPU52_26550) 5489832..5490761 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OPU52_RS26560 (OPU52_26555) 5490935..5491318 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
OPU52_RS26565 (OPU52_26560) 5491318..5491560 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OPU52_RS26570 (OPU52_26565) 5491765..5492682 + 918 WP_064171510.1 alpha/beta hydrolase -
OPU52_RS26575 (OPU52_26570) 5492696..5493637 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
OPU52_RS26580 (OPU52_26575) 5493682..5494119 - 438 WP_008807906.1 D-aminoacyl-tRNA deacylase -
OPU52_RS26585 (OPU52_26580) 5494116..5494976 - 861 WP_008807907.1 virulence factor BrkB family protein -
OPU52_RS26590 (OPU52_26585) 5494970..5495569 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T266624 WP_008807903.1 NZ_CP114361:c5491318-5490935 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT266624 WP_004150355.1 NZ_CP114361:c5491560-5491318 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References