Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1914111..1914662 Replicon chromosome
Accession NZ_CP114361
Organism Klebsiella variicola strain 2022CK-00568

Toxin (Protein)


Gene name pemK Uniprot ID A0A2V3KRZ7
Locus tag OPU52_RS09250 Protein ID WP_046881584.1
Coordinates 1914111..1914443 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A2V3KU66
Locus tag OPU52_RS09255 Protein ID WP_046881585.1
Coordinates 1914444..1914662 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPU52_RS09220 (OPU52_09220) 1909877..1910977 - 1101 WP_070612302.1 AarF/UbiB family protein -
OPU52_RS09225 (OPU52_09225) 1911504..1911761 + 258 WP_012541132.1 antitoxin -
OPU52_RS09230 (OPU52_09230) 1911762..1912094 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin -
OPU52_RS09240 (OPU52_09240) 1912417..1913853 + 1437 WP_269136626.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
OPU52_RS09250 (OPU52_09250) 1914111..1914443 - 333 WP_046881584.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OPU52_RS09255 (OPU52_09255) 1914444..1914662 - 219 WP_046881585.1 antitoxin Antitoxin
OPU52_RS09260 (OPU52_09260) 1914974..1915954 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
OPU52_RS09265 (OPU52_09265) 1916050..1917984 + 1935 WP_046881586.1 P-loop NTPase fold protein -
OPU52_RS09270 (OPU52_09270) 1917984..1918850 + 867 WP_046881587.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1911762..1925681 13919
- flank IS/Tn - - 1914974..1915954 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11751.64 Da        Isoelectric Point: 10.8919

>T266615 WP_046881584.1 NZ_CP114361:c1914443-1914111 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKAAFNKQTRLPVVVPVTSGGNFARATGFTVSLDGVGTKTTGVLRCDQPRTI
VMGARSGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7877.07 Da        Isoelectric Point: 4.1364

>AT266615 WP_046881585.1 NZ_CP114361:c1914662-1914444 [Klebsiella variicola]
MLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIM

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KRZ7

Antitoxin

Source ID Structure
AlphaFold DB A0A2V3KU66

References