Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1911504..1912094 Replicon chromosome
Accession NZ_CP114361
Organism Klebsiella variicola strain 2022CK-00568

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag OPU52_RS09230 Protein ID WP_008804165.1
Coordinates 1911762..1912094 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag OPU52_RS09225 Protein ID WP_012541132.1
Coordinates 1911504..1911761 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPU52_RS09205 (OPU52_09205) 1907112..1907687 + 576 WP_012541129.1 hypothetical protein -
OPU52_RS09210 (OPU52_09210) 1907866..1908702 + 837 WP_008804155.1 alpha/beta hydrolase -
OPU52_RS09215 (OPU52_09215) 1908909..1909880 + 972 WP_016161433.1 sensor domain-containing diguanylate cyclase -
OPU52_RS09220 (OPU52_09220) 1909877..1910977 - 1101 WP_070612302.1 AarF/UbiB family protein -
OPU52_RS09225 (OPU52_09225) 1911504..1911761 + 258 WP_012541132.1 antitoxin Antitoxin
OPU52_RS09230 (OPU52_09230) 1911762..1912094 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OPU52_RS09240 (OPU52_09240) 1912417..1913853 + 1437 WP_269136626.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
OPU52_RS09250 (OPU52_09250) 1914111..1914443 - 333 WP_046881584.1 type II toxin-antitoxin system PemK/MazF family toxin -
OPU52_RS09255 (OPU52_09255) 1914444..1914662 - 219 WP_046881585.1 antitoxin -
OPU52_RS09260 (OPU52_09260) 1914974..1915954 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1914974..1915954 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T266614 WP_008804165.1 NZ_CP114361:1911762-1912094 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT266614 WP_012541132.1 NZ_CP114361:1911504-1911761 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References