Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 301362..301993 Replicon chromosome
Accession NZ_CP114361
Organism Klebsiella variicola strain 2022CK-00568

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag OPU52_RS01365 Protein ID WP_044650325.1
Coordinates 301362..301637 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag OPU52_RS01370 Protein ID WP_004181489.1
Coordinates 301634..301993 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPU52_RS01345 (OPU52_01345) 296598..296933 + 336 WP_002921203.1 universal stress protein UspB -
OPU52_RS01350 (OPU52_01350) 296993..298489 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
OPU52_RS01355 (OPU52_01355) 298719..299912 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
OPU52_RS01360 (OPU52_01360) 299952..300965 - 1014 WP_008806942.1 magnesium transporter -
OPU52_RS01365 (OPU52_01365) 301362..301637 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
OPU52_RS01370 (OPU52_01370) 301634..301993 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OPU52_RS01375 (OPU52_01375) 302021..302422 - 402 WP_032733900.1 nickel-responsive transcriptional regulator NikR -
OPU52_RS01380 (OPU52_01380) 302410..303201 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
OPU52_RS01385 (OPU52_01385) 303198..303962 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
OPU52_RS01390 (OPU52_01390) 303962..304795 - 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
OPU52_RS01395 (OPU52_01395) 304792..305736 - 945 WP_016161924.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T266609 WP_044650325.1 NZ_CP114361:301362-301637 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT266609 WP_004181489.1 NZ_CP114361:301634-301993 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References