Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7369..8012 Replicon plasmid unnamed1
Accession NZ_CP114355
Organism Klebsiella variicola strain 2022CK-00567

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag OPU51_RS26835 Protein ID WP_001044770.1
Coordinates 7596..8012 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag OPU51_RS26830 Protein ID WP_001261282.1
Coordinates 7369..7599 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPU51_RS26805 (OPU51_26805) 2994..3974 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
OPU51_RS26810 (OPU51_26810) 4064..4807 + 744 WP_269776242.1 hypothetical protein -
OPU51_RS26815 (OPU51_26815) 4992..6320 + 1329 WP_004206607.1 nucleotidyltransferase -
OPU51_RS26820 (OPU51_26820) 6328..6903 + 576 WP_004206608.1 SLATT domain-containing protein -
OPU51_RS26825 (OPU51_26825) 7170..7412 - 243 Protein_6 hypothetical protein -
OPU51_RS26830 (OPU51_26830) 7369..7599 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
OPU51_RS26835 (OPU51_26835) 7596..8012 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
OPU51_RS26840 (OPU51_26840) 8086..9648 + 1563 WP_004206609.1 AAA family ATPase -
OPU51_RS26845 (OPU51_26845) 9633..10655 + 1023 WP_000361404.1 helicase UvrD -
OPU51_RS26850 (OPU51_26850) 11199..12107 + 909 WP_032426221.1 HNH endonuclease -
OPU51_RS26855 (OPU51_26855) 12293..12643 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..236560 236560
- flank IS/Tn - - 2994..3974 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T266605 WP_001044770.1 NZ_CP114355:7596-8012 [Klebsiella variicola]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT266605 WP_001261282.1 NZ_CP114355:7369-7599 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References