Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5455314..5455939 Replicon chromosome
Accession NZ_CP114354
Organism Klebsiella variicola strain 2022CK-00567

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag OPU51_RS26430 Protein ID WP_008807903.1
Coordinates 5455314..5455697 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OPU51_RS26435 Protein ID WP_004150355.1
Coordinates 5455697..5455939 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPU51_RS26415 (OPU51_26415) 5452680..5453582 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OPU51_RS26420 (OPU51_26420) 5453579..5454214 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
OPU51_RS26425 (OPU51_26425) 5454211..5455140 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OPU51_RS26430 (OPU51_26430) 5455314..5455697 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
OPU51_RS26435 (OPU51_26435) 5455697..5455939 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OPU51_RS26440 (OPU51_26440) 5456144..5457061 + 918 WP_064171510.1 alpha/beta hydrolase -
OPU51_RS26445 (OPU51_26445) 5457075..5458016 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
OPU51_RS26450 (OPU51_26450) 5458061..5458498 - 438 WP_008807906.1 D-aminoacyl-tRNA deacylase -
OPU51_RS26455 (OPU51_26455) 5458495..5459355 - 861 WP_008807907.1 virulence factor BrkB family protein -
OPU51_RS26460 (OPU51_26460) 5459349..5459948 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T266604 WP_008807903.1 NZ_CP114354:c5455697-5455314 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT266604 WP_004150355.1 NZ_CP114354:c5455939-5455697 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References