Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1911505..1912095 Replicon chromosome
Accession NZ_CP114354
Organism Klebsiella variicola strain 2022CK-00567

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag OPU51_RS09225 Protein ID WP_008804165.1
Coordinates 1911763..1912095 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag OPU51_RS09220 Protein ID WP_012541132.1
Coordinates 1911505..1911762 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPU51_RS09200 (OPU51_09205) 1907113..1907688 + 576 WP_012541129.1 hypothetical protein -
OPU51_RS09205 (OPU51_09210) 1907867..1908703 + 837 WP_008804155.1 alpha/beta hydrolase -
OPU51_RS09210 (OPU51_09215) 1908910..1909881 + 972 WP_016161433.1 sensor domain-containing diguanylate cyclase -
OPU51_RS09215 (OPU51_09220) 1909878..1910978 - 1101 WP_070612302.1 AarF/UbiB family protein -
OPU51_RS09220 (OPU51_09225) 1911505..1911762 + 258 WP_012541132.1 antitoxin Antitoxin
OPU51_RS09225 (OPU51_09230) 1911763..1912095 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OPU51_RS09235 (OPU51_09240) 1912418..1913854 + 1437 WP_269136626.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
OPU51_RS09245 (OPU51_09250) 1914112..1914444 - 333 WP_046881584.1 type II toxin-antitoxin system PemK/MazF family toxin -
OPU51_RS09250 (OPU51_09255) 1914445..1914663 - 219 WP_046881585.1 antitoxin -
OPU51_RS09255 (OPU51_09260) 1914975..1915955 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1914975..1915955 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T266595 WP_008804165.1 NZ_CP114354:1911763-1912095 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT266595 WP_012541132.1 NZ_CP114354:1911505-1911762 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References