Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7369..8012 Replicon plasmid unnamed1
Accession NZ_CP114333
Organism Klebsiella variicola strain 2022CK-00505

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag OGU18_RS26960 Protein ID WP_001044770.1
Coordinates 7596..8012 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag OGU18_RS26955 Protein ID WP_001261282.1
Coordinates 7369..7599 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OGU18_RS26930 (OGU18_26925) 2994..3974 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
OGU18_RS26935 (OGU18_26930) 4064..4807 + 744 WP_269776242.1 hypothetical protein -
OGU18_RS26940 (OGU18_26935) 4992..6320 + 1329 WP_004206607.1 nucleotidyltransferase -
OGU18_RS26945 (OGU18_26940) 6328..6903 + 576 WP_004206608.1 SLATT domain-containing protein -
OGU18_RS26950 (OGU18_26945) 7170..7412 - 243 Protein_6 hypothetical protein -
OGU18_RS26955 (OGU18_26950) 7369..7599 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
OGU18_RS26960 (OGU18_26955) 7596..8012 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
OGU18_RS26965 (OGU18_26960) 8086..9648 + 1563 WP_004206609.1 AAA family ATPase -
OGU18_RS26970 (OGU18_26965) 9633..10655 + 1023 WP_000361404.1 helicase UvrD -
OGU18_RS26975 (OGU18_26970) 11199..12107 + 909 WP_032426221.1 HNH endonuclease -
OGU18_RS26980 (OGU18_26975) 12293..12643 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..227788 227788
- flank IS/Tn - - 2994..3974 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T266587 WP_001044770.1 NZ_CP114333:7596-8012 [Klebsiella variicola]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT266587 WP_001261282.1 NZ_CP114333:7369-7599 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References