Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 301363..301994 Replicon chromosome
Accession NZ_CP114320
Organism Klebsiella variicola strain 2022CK-00502

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag OEE46_RS01365 Protein ID WP_044650325.1
Coordinates 301363..301638 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag OEE46_RS01370 Protein ID WP_004181489.1
Coordinates 301635..301994 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OEE46_RS01345 (OEE46_01345) 296599..296934 + 336 WP_002921203.1 universal stress protein UspB -
OEE46_RS01350 (OEE46_01350) 296994..298490 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
OEE46_RS01355 (OEE46_01355) 298720..299913 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
OEE46_RS01360 (OEE46_01360) 299953..300966 - 1014 WP_008806942.1 magnesium transporter -
OEE46_RS01365 (OEE46_01365) 301363..301638 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
OEE46_RS01370 (OEE46_01370) 301635..301994 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OEE46_RS01375 (OEE46_01375) 302022..302423 - 402 WP_032733900.1 nickel-responsive transcriptional regulator NikR -
OEE46_RS01380 (OEE46_01380) 302411..303202 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
OEE46_RS01385 (OEE46_01385) 303199..303963 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
OEE46_RS01390 (OEE46_01390) 303963..304796 - 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
OEE46_RS01395 (OEE46_01395) 304793..305737 - 945 WP_016161924.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T266514 WP_044650325.1 NZ_CP114320:301363-301638 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT266514 WP_004181489.1 NZ_CP114320:301635-301994 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References