Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 69851..70587 Replicon plasmid unnamed1
Accession NZ_CP114169
Organism Klebsiella variicola strain 2022CK-00565

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag OIX89_RS27285 Protein ID WP_003026803.1
Coordinates 70105..70587 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag OIX89_RS27280 Protein ID WP_003026799.1
Coordinates 69851..70117 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIX89_RS27245 (OIX89_27245) 65695..66057 - 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -
OIX89_RS27250 (OIX89_27250) 66109..66459 - 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
OIX89_RS27255 (OIX89_27255) 66817..67065 + 249 WP_004193994.1 hypothetical protein -
OIX89_RS27260 (OIX89_27260) 67062..68273 + 1212 WP_032415728.1 hypothetical protein -
OIX89_RS27265 (OIX89_27265) 68407..68898 + 492 WP_004206662.1 hypothetical protein -
OIX89_RS27270 (OIX89_27270) 68959..69162 + 204 WP_004206661.1 HHA domain-containing protein -
OIX89_RS27275 (OIX89_27275) 69176..69406 + 231 WP_004206660.1 hypothetical protein -
OIX89_RS27280 (OIX89_27280) 69851..70117 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
OIX89_RS27285 (OIX89_27285) 70105..70587 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
OIX89_RS27290 (OIX89_27290) 70788..72191 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
OIX89_RS27295 (OIX89_27295) 72220..72852 - 633 WP_001567369.1 hypothetical protein -
OIX89_RS27300 (OIX89_27300) 73078..74424 + 1347 WP_064152331.1 ISNCY family transposase -
OIX89_RS27305 (OIX89_27305) 74473..74868 + 396 WP_048268429.1 helix-turn-helix domain-containing protein -
OIX89_RS27310 (OIX89_27310) 75021..75131 - 111 Protein_79 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..239010 239010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T266404 WP_003026803.1 NZ_CP114169:70105-70587 [Klebsiella variicola]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT266404 WP_003026799.1 NZ_CP114169:69851-70117 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References