Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3541494..3542045 Replicon chromosome
Accession NZ_CP114168
Organism Klebsiella variicola strain 2022CK-00565

Toxin (Protein)


Gene name pemK Uniprot ID A0A2V3KRZ7
Locus tag OIX89_RS16960 Protein ID WP_046881584.1
Coordinates 3541494..3541826 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A2V3KU66
Locus tag OIX89_RS16965 Protein ID WP_046881585.1
Coordinates 3541827..3542045 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIX89_RS16930 (OIX89_16930) 3537260..3538360 - 1101 WP_070612302.1 AarF/UbiB family protein -
OIX89_RS16935 (OIX89_16935) 3538887..3539144 + 258 WP_012541132.1 antitoxin -
OIX89_RS16940 (OIX89_16940) 3539145..3539477 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin -
OIX89_RS16950 (OIX89_16950) 3539800..3541236 + 1437 WP_269136626.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
OIX89_RS16960 (OIX89_16960) 3541494..3541826 - 333 WP_046881584.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OIX89_RS16965 (OIX89_16965) 3541827..3542045 - 219 WP_046881585.1 antitoxin Antitoxin
OIX89_RS16970 (OIX89_16970) 3542357..3543337 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
OIX89_RS16975 (OIX89_16975) 3543433..3545367 + 1935 WP_046881586.1 P-loop NTPase fold protein -
OIX89_RS16980 (OIX89_16980) 3545367..3546233 + 867 WP_046881587.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3539145..3553064 13919
- flank IS/Tn - - 3542357..3543337 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11751.64 Da        Isoelectric Point: 10.8919

>T266397 WP_046881584.1 NZ_CP114168:c3541826-3541494 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKAAFNKQTRLPVVVPVTSGGNFARATGFTVSLDGVGTKTTGVLRCDQPRTI
VMGARSGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7877.07 Da        Isoelectric Point: 4.1364

>AT266397 WP_046881585.1 NZ_CP114168:c3542045-3541827 [Klebsiella variicola]
MLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIM

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KRZ7

Antitoxin

Source ID Structure
AlphaFold DB A0A2V3KU66

References