Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 3351454..3352451 Replicon chromosome
Accession NZ_CP114168
Organism Klebsiella variicola strain 2022CK-00565

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag OIX89_RS16125 Protein ID WP_229531768.1
Coordinates 3351897..3352451 (-) Length 185 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A1F2MDT5
Locus tag OIX89_RS16120 Protein ID WP_012541033.1
Coordinates 3351454..3351900 (-) Length 149 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIX89_RS16105 (OIX89_16105) 3347749..3348747 + 999 WP_008804028.1 galactose/glucose ABC transporter substrate-binding protein MglB -
OIX89_RS16110 (OIX89_16110) 3348866..3350386 + 1521 WP_008804029.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
OIX89_RS16115 (OIX89_16115) 3350402..3351412 + 1011 WP_002912871.1 galactose/methyl galactoside ABC transporter permease MglC -
OIX89_RS16120 (OIX89_16120) 3351454..3351900 - 447 WP_012541033.1 helix-turn-helix transcriptional regulator Antitoxin
OIX89_RS16125 (OIX89_16125) 3351897..3352451 - 555 WP_229531768.1 HipA domain-containing protein Toxin
OIX89_RS16130 (OIX89_16130) 3352480..3353001 - 522 WP_025714688.1 hypothetical protein -
OIX89_RS16135 (OIX89_16135) 3353116..3353847 - 732 WP_008804033.1 outer membrane permeability protein SanA -
OIX89_RS16140 (OIX89_16140) 3354140..3355024 - 885 WP_262266825.1 cytidine deaminase -
OIX89_RS16145 (OIX89_16145) 3355153..3355848 - 696 WP_061153873.1 CidB/LrgB family autolysis modulator -
OIX89_RS16150 (OIX89_16150) 3355838..3356242 - 405 WP_004201620.1 CidA/LrgA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(2-147)

Antitoxin

Cro/C1-type helix-turn-helix domain

(78-132)


Sequences

Toxin        


Download         Length: 185 a.a.        Molecular weight: 20710.03 Da        Isoelectric Point: 5.7891

>T266395 WP_229531768.1 NZ_CP114168:c3352451-3351897 [Klebsiella variicola]
MRLGMESVYSLLQKAPATILDHETTLRILIEKITSSNMVRVQNYRFDVQGFIIDWVRRDLLNIIFGNSDNHGRNTAFMKA
DNEIILAPIYDFAPMKADPEGIPRTMKWSLACESGGEYNFGAIAQALAEWITPDTLLNALSETASQLVDLPSRLKVRGVP
VQILEMPSIGFKFIPDKLARWGLL

Download         Length: 555 bp

Antitoxin


Download         Length: 149 a.a.        Molecular weight: 16396.05 Da        Isoelectric Point: 10.5417

>AT266395 WP_012541033.1 NZ_CP114168:c3351900-3351454 [Klebsiella variicola]
MKTLNKKETEIQNTLARLRADNPPSLPATGVTATEKKARSEISRQRVSAGLKKVKTVDRQAVIHAIIHDIMLGAISQGEA
LKKLRVEVLGLRQDEYARLVDVSRKTLSDVENDKGNYSAEIINKIYKPFGLETGLVPISKTLISSLFK

Download         Length: 447 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MDT5

References