Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1929945..1930576 Replicon chromosome
Accession NZ_CP114168
Organism Klebsiella variicola strain 2022CK-00565

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag OIX89_RS09085 Protein ID WP_044650325.1
Coordinates 1929945..1930220 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag OIX89_RS09090 Protein ID WP_004181489.1
Coordinates 1930217..1930576 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIX89_RS09065 (OIX89_09065) 1925181..1925516 + 336 WP_002921203.1 universal stress protein UspB -
OIX89_RS09070 (OIX89_09070) 1925576..1927072 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
OIX89_RS09075 (OIX89_09075) 1927302..1928495 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
OIX89_RS09080 (OIX89_09080) 1928535..1929548 - 1014 WP_008806942.1 magnesium transporter -
OIX89_RS09085 (OIX89_09085) 1929945..1930220 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
OIX89_RS09090 (OIX89_09090) 1930217..1930576 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OIX89_RS09095 (OIX89_09095) 1930604..1931005 - 402 WP_032733900.1 nickel-responsive transcriptional regulator NikR -
OIX89_RS09100 (OIX89_09100) 1930993..1931784 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
OIX89_RS09105 (OIX89_09105) 1931781..1932545 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
OIX89_RS09110 (OIX89_09110) 1932545..1933378 - 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
OIX89_RS09115 (OIX89_09115) 1933375..1934319 - 945 WP_016161924.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T266391 WP_044650325.1 NZ_CP114168:1929945-1930220 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT266391 WP_004181489.1 NZ_CP114168:1930217-1930576 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References