Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1550681..1551306 Replicon chromosome
Accession NZ_CP114168
Organism Klebsiella variicola strain 2022CK-00565

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag OIX89_RS07360 Protein ID WP_008807903.1
Coordinates 1550681..1551064 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OIX89_RS07365 Protein ID WP_004150355.1
Coordinates 1551064..1551306 (-) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIX89_RS07345 (OIX89_07345) 1548047..1548949 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OIX89_RS07350 (OIX89_07350) 1548946..1549581 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
OIX89_RS07355 (OIX89_07355) 1549578..1550507 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OIX89_RS07360 (OIX89_07360) 1550681..1551064 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
OIX89_RS07365 (OIX89_07365) 1551064..1551306 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OIX89_RS07370 (OIX89_07370) 1551511..1552428 + 918 WP_064171510.1 alpha/beta hydrolase -
OIX89_RS07375 (OIX89_07375) 1552442..1553383 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
OIX89_RS07380 (OIX89_07380) 1553428..1553865 - 438 WP_008807906.1 D-aminoacyl-tRNA deacylase -
OIX89_RS07385 (OIX89_07385) 1553862..1554722 - 861 WP_008807907.1 virulence factor BrkB family protein -
OIX89_RS07390 (OIX89_07390) 1554716..1555315 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-112)

Antitoxin

(16-47)


Sequences


Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T266390 WP_008807903.1 NZ_CP114168:c1551064-1550681 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp


Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT266390 WP_004150355.1 NZ_CP114168:c1551306-1551064 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1F2M041


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References