Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 83220..83956 Replicon plasmid unnamed1
Accession NZ_CP114161
Organism Klebsiella quasipneumoniae strain 2022CK-00516

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag N5B28_RS26240 Protein ID WP_003026803.1
Coordinates 83474..83956 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag N5B28_RS26235 Protein ID WP_003026799.1
Coordinates 83220..83486 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5B28_RS26210 (N5B28_26210) 78686..79759 + 1074 WP_017900354.1 urea ABC transporter permease subunit UrtC -
N5B28_RS26215 (N5B28_26215) 79759..80556 + 798 WP_004205978.1 urea ABC transporter ATP-binding protein UrtD -
N5B28_RS26220 (N5B28_26220) 80566..81264 + 699 WP_004205977.1 urea ABC transporter ATP-binding subunit UrtE -
N5B28_RS26225 (N5B28_26225) 81300..81782 - 483 WP_004205976.1 helix-turn-helix domain-containing protein -
N5B28_RS26230 (N5B28_26230) 81842..82810 + 969 WP_072202995.1 IS5 family transposase -
N5B28_RS26235 (N5B28_26235) 83220..83486 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
N5B28_RS26240 (N5B28_26240) 83474..83956 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
N5B28_RS26245 (N5B28_26245) 84168..85514 + 1347 WP_077251107.1 ISNCY family transposase -
N5B28_RS26250 (N5B28_26250) 85674..86378 + 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -
N5B28_RS26255 (N5B28_26255) 86713..86970 - 258 WP_023292103.1 hypothetical protein -
N5B28_RS26260 (N5B28_26260) 87613..88128 + 516 Protein_95 CSS-motif domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..175750 175750
- inside IScluster/Tn - - 81842..85514 3672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T266365 WP_003026803.1 NZ_CP114161:83474-83956 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT266365 WP_003026799.1 NZ_CP114161:83220-83486 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References