Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5134673..5135249 Replicon chromosome
Accession NZ_CP114156
Organism Klebsiella michiganensis strain 2022CK-00497

Toxin (Protein)


Gene name shpA Uniprot ID A0A443WNM0
Locus tag OEE44_RS24235 Protein ID WP_064380994.1
Coordinates 5134962..5135249 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0H3H591
Locus tag OEE44_RS24230 Protein ID WP_014227757.1
Coordinates 5134673..5134975 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OEE44_RS24215 (OEE44_24215) 5131512..5131847 + 336 WP_014227760.1 endoribonuclease SymE -
OEE44_RS24220 (OEE44_24220) 5132301..5133212 + 912 WP_182094964.1 acetamidase/formamidase family protein -
OEE44_RS24225 (OEE44_24225) 5133209..5134552 + 1344 WP_004128506.1 APC family permease -
OEE44_RS24230 (OEE44_24230) 5134673..5134975 - 303 WP_014227757.1 BrnA antitoxin family protein Antitoxin
OEE44_RS24235 (OEE44_24235) 5134962..5135249 - 288 WP_064380994.1 BrnT family toxin Toxin
OEE44_RS24240 (OEE44_24240) 5135509..5135952 - 444 WP_014227755.1 FosA family fosfomycin resistance glutathione transferase -
OEE44_RS24245 (OEE44_24245) 5135946..5136854 - 909 WP_049080873.1 LysR family transcriptional regulator -
OEE44_RS24250 (OEE44_24250) 5136942..5137724 + 783 WP_025108473.1 NAD(P)H-dependent oxidoreductase -
OEE44_RS24255 (OEE44_24255) 5137872..5138456 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
OEE44_RS24260 (OEE44_24260) 5138474..5139274 + 801 WP_182094965.1 winged helix-turn-helix domain-containing protein -
OEE44_RS24265 (OEE44_24265) 5139271..5139789 + 519 WP_004098240.1 FidL-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11162.65 Da        Isoelectric Point: 7.4687

>T266351 WP_064380994.1 NZ_CP114156:c5135249-5134962 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVLDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11607.18 Da        Isoelectric Point: 9.4429

>AT266351 WP_014227757.1 NZ_CP114156:c5134975-5134673 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443WNM0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H591

References