Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56829..57376 Replicon chromosome
Accession NZ_CP114156
Organism Klebsiella michiganensis strain 2022CK-00497

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag OEE44_RS00285 Protein ID WP_014227299.1
Coordinates 56829..57137 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag OEE44_RS00290 Protein ID WP_014227298.1
Coordinates 57140..57376 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OEE44_RS00260 (OEE44_00260) 52745..53056 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
OEE44_RS00265 (OEE44_00265) 53351..54292 + 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
OEE44_RS00270 (OEE44_00270) 54316..54609 - 294 WP_014227302.1 YicS family protein -
OEE44_RS00275 (OEE44_00275) 54819..55772 + 954 WP_032751828.1 Rpn family recombination-promoting nuclease/putative transposase -
OEE44_RS00280 (OEE44_00280) 55776..56678 - 903 WP_014227300.1 EamA family transporter -
OEE44_RS00285 (OEE44_00285) 56829..57137 - 309 WP_014227299.1 CcdB family protein Toxin
OEE44_RS00290 (OEE44_00290) 57140..57376 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
OEE44_RS00295 (OEE44_00295) 57482..58915 - 1434 WP_014839829.1 PTS N-acetylmuramic acid transporter subunit IIBC -
OEE44_RS00300 (OEE44_00300) 58940..59842 - 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
OEE44_RS00305 (OEE44_00305) 60003..61169 - 1167 WP_085520169.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T266342 WP_014227299.1 NZ_CP114156:c57137-56829 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT266342 WP_014227298.1 NZ_CP114156:c57376-57140 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References