Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 25384..26294 Replicon plasmid unnamed1
Accession NZ_CP114150
Organism Klebsiella michiganensis strain 2022CK-00496

Toxin (Protein)


Gene name - Uniprot ID A0A2A5MBW7
Locus tag OEE45_RS28210 Protein ID WP_023329005.1
Coordinates 25824..26294 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID -
Locus tag OEE45_RS28205 Protein ID WP_110184558.1
Coordinates 25384..25827 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OEE45_RS28180 (OEE45_28190) 21276..21506 - 231 Protein_28 hypothetical protein -
OEE45_RS28185 (OEE45_28195) 21705..22426 + 722 Protein_29 DUF969 domain-containing protein -
OEE45_RS28190 (OEE45_28200) 22423..23409 + 987 Protein_30 DUF979 domain-containing protein -
OEE45_RS28195 (OEE45_28205) 23419..24414 + 996 WP_049084154.1 DUF2891 domain-containing protein -
OEE45_RS28200 (OEE45_28210) 24596..25262 + 667 Protein_32 DUF4113 domain-containing protein -
OEE45_RS28205 (OEE45_28215) 25384..25827 + 444 WP_110184558.1 DUF2384 domain-containing protein Antitoxin
OEE45_RS28210 (OEE45_28220) 25824..26294 + 471 WP_023329005.1 RES family NAD+ phosphorylase Toxin
OEE45_RS28215 (OEE45_28225) 26476..26664 - 189 WP_023329006.1 hypothetical protein -
OEE45_RS28220 (OEE45_28230) 27359..28734 + 1376 Protein_36 IS3 family transposase -
OEE45_RS28225 (OEE45_28235) 28983..29558 - 576 WP_110184559.1 cytochrome b/b6 domain-containing protein -
OEE45_RS28230 (OEE45_28240) 29706..30011 + 306 WP_023328906.1 metalloregulator ArsR/SmtB family transcription factor -
OEE45_RS28235 (OEE45_28245) 30013..30529 + 517 Protein_39 rhodanese-like domain-containing protein -
OEE45_RS28240 (OEE45_28250) 30722..30871 - 150 Protein_40 cation-efflux pump -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..186939 186939
- inside IScluster/Tn - - 27976..40621 12645


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(94-144)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17459.70 Da        Isoelectric Point: 4.4829

>T266338 WP_023329005.1 NZ_CP114150:25824-26294 [Klebsiella michiganensis]
VILYRLTKTKNLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPANWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHSDFYGIVQMAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16461.82 Da        Isoelectric Point: 10.2498

>AT266338 WP_110184558.1 NZ_CP114150:25384-25827 [Klebsiella michiganensis]
MKTFSLSSTPARPQRLWQIAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAALKWLNEPNRALSWKVPADLIASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBW7

Antitoxin

Source ID Structure

References