Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5257823..5258399 Replicon chromosome
Accession NZ_CP114149
Organism Klebsiella michiganensis strain 2022CK-00496

Toxin (Protein)


Gene name shpA Uniprot ID A0A1Q8YXR6
Locus tag OEE45_RS24775 Protein ID WP_046878034.1
Coordinates 5258112..5258399 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0H3H591
Locus tag OEE45_RS24770 Protein ID WP_014227757.1
Coordinates 5257823..5258125 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OEE45_RS24755 (OEE45_24765) 5254659..5254994 + 336 WP_064375703.1 endoribonuclease SymE -
OEE45_RS24760 (OEE45_24770) 5255451..5256362 + 912 WP_049080536.1 acetamidase/formamidase family protein -
OEE45_RS24765 (OEE45_24775) 5256359..5257702 + 1344 WP_049101852.1 APC family permease -
OEE45_RS24770 (OEE45_24780) 5257823..5258125 - 303 WP_014227757.1 BrnA antitoxin family protein Antitoxin
OEE45_RS24775 (OEE45_24785) 5258112..5258399 - 288 WP_046878034.1 BrnT family toxin Toxin
OEE45_RS24780 (OEE45_24790) 5258554..5259522 - 969 WP_016151359.1 IS5-like element IS903B family transposase -
OEE45_RS24785 (OEE45_24795) 5259717..5260160 - 444 WP_014227755.1 FosA family fosfomycin resistance glutathione transferase -
OEE45_RS24790 (OEE45_24800) 5260154..5261062 - 909 WP_064375700.1 LysR family transcriptional regulator -
OEE45_RS24795 (OEE45_24805) 5261150..5261932 + 783 WP_064358753.1 NAD(P)H-dependent oxidoreductase -
OEE45_RS24800 (OEE45_24810) 5262080..5262664 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn fosA - 5258554..5260160 1606


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11210.69 Da        Isoelectric Point: 7.4687

>T266337 WP_046878034.1 NZ_CP114149:c5258399-5258112 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11607.18 Da        Isoelectric Point: 9.4429

>AT266337 WP_014227757.1 NZ_CP114149:c5258125-5257823 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YXR6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H591

References