Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4355659..4356185 Replicon chromosome
Accession NZ_CP114149
Organism Klebsiella michiganensis strain 2022CK-00496

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag OEE45_RS20655 Protein ID WP_000323025.1
Coordinates 4355659..4355946 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag OEE45_RS20660 Protein ID WP_000534858.1
Coordinates 4355946..4356185 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OEE45_RS20630 (OEE45_20630) 4350809..4352353 + 1545 WP_014228343.1 sugar ABC transporter ATP-binding protein -
OEE45_RS20635 (OEE45_20635) 4352371..4353426 + 1056 WP_004848640.1 ABC transporter permease -
OEE45_RS20640 (OEE45_20640) 4353487..4354284 + 798 WP_014228342.1 SDR family oxidoreductase -
OEE45_RS20645 (OEE45_20645) 4354464..4355249 + 786 WP_064358087.1 SDR family oxidoreductase -
OEE45_RS20650 (OEE45_20650) 4355444..4355596 - 153 WP_004130292.1 Hok/Gef family protein -
OEE45_RS20655 (OEE45_20655) 4355659..4355946 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
OEE45_RS20660 (OEE45_20660) 4355946..4356185 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
OEE45_RS20665 (OEE45_20665) 4356210..4356314 + 105 Protein_4063 protein YdfV -
OEE45_RS20670 (OEE45_20670) 4356448..4357371 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
OEE45_RS20675 (OEE45_20675) 4357571..4358143 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
OEE45_RS20680 (OEE45_20680) 4358786..4360126 - 1341 WP_000589001.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4360201..4361733 1532


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T266334 WP_000323025.1 NZ_CP114149:c4355946-4355659 [Klebsiella michiganensis]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT266334 WP_000534858.1 NZ_CP114149:c4356185-4355946 [Klebsiella michiganensis]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References