Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4596964..4597461 Replicon chromosome
Accession NZ_CP114130
Organism Escherichia coli strain NJS001

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N0F11_RS22880 Protein ID WP_001173343.1
Coordinates 4596964..4597137 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N0F11_RS22885 Protein ID WP_000398619.1
Coordinates 4597165..4597461 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0F11_RS22870 (4593634) 4593634..4595091 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
N0F11_RS22875 (4595328) 4595328..4596845 + 1518 WP_001295074.1 lysine--tRNA ligase -
N0F11_RS22880 (4596964) 4596964..4597137 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N0F11_RS22885 (4597165) 4597165..4597461 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N0F11_RS22890 (4597688) 4597688..4597960 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N0F11_RS22895 (4597972) 4597972..4598202 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N0F11_RS22900 (4598383) 4598383..4600014 + 1632 WP_001216477.1 sensor histidine kinase -
N0F11_RS22905 (4600011) 4600011..4600730 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T266316 WP_001173343.1 NZ_CP114130:c4597137-4596964 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT266316 WP_000398619.1 NZ_CP114130:c4597461-4597165 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References