Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 517604..518240 Replicon chromosome
Accession NZ_CP114066
Organism Bacillus halotolerans strain B13

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag O0R52_RS02645 Protein ID WP_003156187.1
Coordinates 517890..518240 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag O0R52_RS02640 Protein ID WP_003225183.1
Coordinates 517604..517885 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O0R52_RS02620 (O0R52_02620) 513961..514560 - 600 WP_269107820.1 rhomboid family intramembrane serine protease -
O0R52_RS02625 (O0R52_02625) 514655..515020 + 366 WP_256766654.1 holo-ACP synthase -
O0R52_RS02630 (O0R52_02630) 515186..516202 + 1017 WP_256766957.1 outer membrane lipoprotein carrier protein LolA -
O0R52_RS02635 (O0R52_02635) 516319..517488 + 1170 WP_044161733.1 alanine racemase -
O0R52_RS02640 (O0R52_02640) 517604..517885 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
O0R52_RS02645 (O0R52_02645) 517890..518240 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
O0R52_RS02650 (O0R52_02650) 518356..519180 + 825 WP_082687807.1 RsbT co-antagonist protein RsbRA -
O0R52_RS02655 (O0R52_02655) 519185..519550 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
O0R52_RS02660 (O0R52_02660) 519554..519955 + 402 WP_024714447.1 serine/threonine-protein kinase RsbT -
O0R52_RS02665 (O0R52_02665) 519967..520974 + 1008 WP_044161721.1 phosphoserine phosphatase RsbU -
O0R52_RS02670 (O0R52_02670) 521035..521364 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
O0R52_RS02675 (O0R52_02675) 521361..521843 + 483 WP_024120302.1 anti-sigma B factor RsbW -
O0R52_RS02680 (O0R52_02680) 521809..522597 + 789 WP_024120303.1 RNA polymerase sigma factor SigB -
O0R52_RS02685 (O0R52_02685) 522597..523196 + 600 WP_217828106.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T266248 WP_003156187.1 NZ_CP114066:517890-518240 [Bacillus halotolerans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT266248 WP_003225183.1 NZ_CP114066:517604-517885 [Bacillus halotolerans]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References