Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 3152635..3153552 Replicon chromosome
Accession NZ_CP114038
Organism Bacillus velezensis strain B8

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag OZA25_RS15380 Protein ID WP_007407256.1
Coordinates 3152635..3153381 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag OZA25_RS15385 Protein ID WP_003154807.1
Coordinates 3153382..3153552 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OZA25_RS15360 (OZA25_15360) 3148028..3148978 - 951 WP_032874618.1 ring-cleaving dioxygenase -
OZA25_RS15365 (OZA25_15365) 3149300..3150616 + 1317 WP_032874616.1 amino acid permease -
OZA25_RS15370 (OZA25_15370) 3150902..3151519 + 618 WP_032874614.1 DUF47 domain-containing protein -
OZA25_RS15375 (OZA25_15375) 3151532..3152530 + 999 WP_032874611.1 inorganic phosphate transporter -
OZA25_RS15380 (OZA25_15380) 3152635..3153381 + 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
OZA25_RS15385 (OZA25_15385) 3153382..3153552 + 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
OZA25_RS15390 (OZA25_15390) 3153649..3153774 + 126 WP_003154809.1 hypothetical protein -
OZA25_RS15395 (OZA25_15395) 3153809..3154687 - 879 WP_032874609.1 N-acetylmuramoyl-L-alanine amidase -
OZA25_RS15400 (OZA25_15400) 3154701..3154964 - 264 WP_003154813.1 phage holin -
OZA25_RS15405 (OZA25_15405) 3154978..3155241 - 264 WP_003154815.1 hemolysin XhlA family protein -
OZA25_RS15410 (OZA25_15410) 3155293..3156054 - 762 WP_032874607.1 hypothetical protein -
OZA25_RS15415 (OZA25_15415) 3156111..3156308 - 198 WP_032874605.1 XkdX family protein -
OZA25_RS15420 (OZA25_15420) 3156313..3156684 - 372 WP_032874603.1 XkdW family protein -
OZA25_RS15425 (OZA25_15425) 3156697..3158319 - 1623 WP_032874601.1 pyocin knob domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3144360..3197306 52946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T266222 WP_007407256.1 NZ_CP114038:3152635-3153381 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT266222 WP_003154807.1 NZ_CP114038:3153382-3153552 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References