Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 90281..91849 Replicon plasmid pPSKoxy4_2
Accession NZ_CP113906
Organism Klebsiella michiganensis strain PS_Koxy4

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag F0A14_RS30130 Protein ID WP_024134513.1
Coordinates 90542..91849 (+) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag F0A14_RS30125 Protein ID WP_039272366.1
Coordinates 90281..90532 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0A14_RS30095 (F0A14_030090) 85934..86659 + 726 WP_001553093.1 hypothetical protein -
F0A14_RS30100 (F0A14_030095) 86715..87167 + 453 WP_001553092.1 hypothetical protein -
F0A14_RS30105 (F0A14_030100) 87183..87785 - 603 WP_039272372.1 hypothetical protein -
F0A14_RS30110 (F0A14_030105) 88046..88327 + 282 WP_000575656.1 hypothetical protein -
F0A14_RS30115 (F0A14_030110) 88346..88909 + 564 WP_001132404.1 hypothetical protein -
F0A14_RS30120 (F0A14_030115) 88912..89916 + 1005 WP_039272368.1 tyrosine-type recombinase/integrase -
F0A14_RS30125 (F0A14_030120) 90281..90532 + 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
F0A14_RS30130 (F0A14_030125) 90542..91849 + 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
F0A14_RS30135 (F0A14_030130) 91846..92340 + 495 WP_039272364.1 hypothetical protein -
F0A14_RS30140 (F0A14_030135) 92591..93295 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
F0A14_RS30145 (F0A14_030140) 93347..94261 + 915 Protein_113 IS110-like element IS4321 family transposase -
F0A14_RS30150 (F0A14_030145) 94663..95703 + 1041 WP_000101570.1 DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..109858 109858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T266149 WP_024134513.1 NZ_CP113906:90542-91849 [Klebsiella michiganensis]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT266149 WP_039272366.1 NZ_CP113906:90281-90532 [Klebsiella michiganensis]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References