Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5170788..5171364 Replicon chromosome
Accession NZ_CP113904
Organism Klebsiella michiganensis strain PS_Koxy4

Toxin (Protein)


Gene name shpA Uniprot ID A0A443WNM0
Locus tag F0A14_RS24415 Protein ID WP_064380994.1
Coordinates 5171077..5171364 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0H3H591
Locus tag F0A14_RS24410 Protein ID WP_014227757.1
Coordinates 5170788..5171090 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0A14_RS24395 (F0A14_024395) 5167627..5167962 + 336 WP_014227760.1 endoribonuclease SymE -
F0A14_RS24400 (F0A14_024400) 5168416..5169327 + 912 WP_182094964.1 acetamidase/formamidase family protein -
F0A14_RS24405 (F0A14_024405) 5169324..5170667 + 1344 WP_004128506.1 APC family permease -
F0A14_RS24410 (F0A14_024410) 5170788..5171090 - 303 WP_014227757.1 BrnA antitoxin family protein Antitoxin
F0A14_RS24415 (F0A14_024415) 5171077..5171364 - 288 WP_064380994.1 BrnT family toxin Toxin
F0A14_RS24420 (F0A14_024420) 5171624..5172067 - 444 WP_014227755.1 FosA family fosfomycin resistance glutathione transferase -
F0A14_RS24425 (F0A14_024425) 5172061..5172969 - 909 WP_049080873.1 LysR family transcriptional regulator -
F0A14_RS24430 (F0A14_024430) 5173057..5173839 + 783 WP_025108473.1 NAD(P)H-dependent oxidoreductase -
F0A14_RS24435 (F0A14_024435) 5173987..5174571 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
F0A14_RS24440 (F0A14_024440) 5174589..5175389 + 801 WP_182094965.1 winged helix-turn-helix domain-containing protein -
F0A14_RS24445 (F0A14_024445) 5175386..5175904 + 519 WP_004098240.1 FidL-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11162.65 Da        Isoelectric Point: 7.4687

>T266146 WP_064380994.1 NZ_CP113904:c5171364-5171077 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVLDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11607.18 Da        Isoelectric Point: 9.4429

>AT266146 WP_014227757.1 NZ_CP113904:c5171090-5170788 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443WNM0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H591

References