266131

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 21424..22043 Replicon plasmid pEFM61-2
Accession NZ_CP113834
Organism Enterococcus faecalis strain M61

Toxin (Protein)


Gene name mazF Uniprot ID R3H4V2
Locus tag OZF29_RS13990 Protein ID WP_000241511.1
Coordinates 21424..21786 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3H5D1
Locus tag OZF29_RS13995 Protein ID WP_000245205.1
Coordinates 21780..22043 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OZF29_RS13975 (16834) 16834..18765 - 1932 WP_010816308.1 sucrose-specific PTS transporter subunit IIBC -
OZF29_RS13980 (18956) 18956..20395 + 1440 WP_002367771.1 sucrose-6-phosphate hydrolase -
OZF29_RS13985 (20397) 20397..21359 + 963 WP_002367770.1 LacI family DNA-binding transcriptional regulator -
OZF29_RS13990 (21424) 21424..21786 - 363 WP_000241511.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OZF29_RS13995 (21780) 21780..22043 - 264 WP_000245205.1 PbsX family transcriptional regulator Antitoxin
OZF29_RS14000 (22541) 22541..23191 - 651 WP_251846817.1 transposase -
OZF29_RS14005 (24442) 24442..25083 - 642 WP_000406546.1 hypothetical protein -
OZF29_RS14010 (25465) 25465..26778 - 1314 WP_002383787.1 hypothetical protein -
OZF29_RS14015 (26768) 26768..26899 - 132 WP_000159353.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - prgB/asc10 1..68512 68512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13585.78 Da        Isoelectric Point: 9.6107

>T266131 WP_000241511.1 NZ_CP113834:c21786-21424 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Download         Length: 363 bp

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10059.46 Da        Isoelectric Point: 5.1585

>AT266131 WP_000245205.1 NZ_CP113834:c22043-21780 [Enterococcus faecalis]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Download         Length: 264 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3H4V2

Antitoxin

Source ID Structure
AlphaFold DB R3H5D1

References