266130

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 3103..3674 Replicon plasmid pEFM61-2
Accession NZ_CP113834
Organism Enterococcus faecalis strain M61

Toxin (Protein)


Gene name mazF Uniprot ID A0A2P6BPI5
Locus tag OZF29_RS13910 Protein ID WP_002394791.1
Coordinates 3333..3674 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag OZF29_RS13905 Protein ID WP_002362431.1
Coordinates 3103..3333 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OZF29_RS13875 (191) 191..508 + 318 WP_002394802.1 heavy metal-binding domain-containing protein -
OZF29_RS13880 (544) 544..1212 + 669 WP_002403283.1 CPBP family intramembrane metalloprotease -
OZF29_RS13885 (1329) 1329..1583 + 255 WP_002394800.1 hypothetical protein -
OZF29_RS13890 (1743) 1743..1976 - 234 WP_002394799.1 hypothetical protein -
OZF29_RS13895 (1978) 1978..2289 - 312 WP_251846816.1 hypothetical protein -
OZF29_RS13900 (2279) 2279..2899 - 621 WP_002367784.1 recombinase family protein -
OZF29_RS13905 (3103) 3103..3333 + 231 WP_002362431.1 hypothetical protein Antitoxin
OZF29_RS13910 (3333) 3333..3674 + 342 WP_002394791.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OZF29_RS13915 (3786) 3786..4724 + 939 WP_002394789.1 hypothetical protein -
OZF29_RS13920 (4992) 4992..5594 + 603 WP_002367780.1 Fic family protein -
OZF29_RS13925 (5828) 5828..6508 - 681 WP_104680861.1 IS6 family transposase -
OZF29_RS13930 (6763) 6763..7701 + 939 WP_116495287.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - prgB/asc10 1..68512 68512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13248.49 Da        Isoelectric Point: 8.8595

>T266130 WP_002394791.1 NZ_CP113834:3333-3674 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT266130 WP_002362431.1 NZ_CP113834:3103-3333 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P6BPI5

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References