Detailed information of TA system TA026613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2135420..2135949
• Replicon: chromosome
• Accession: NC_017338
• Organism: Staphylococcus aureus subsp. aureus JKD6159

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAA6159_RS10700
• Protein ID: WP_000621175.1
• Coordinates: 2135420..2135782 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAA6159_RS10705
• Protein ID: WP_000948331.1
• Coordinates: 2135779..2135949 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAA6159_RS10675	2132398..2133168	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SAA6159_RS10680	2133143..2133622	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SAA6159_RS10685	2133624..2133950	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAA6159_RS10690	2134069..2135070	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAA6159_RS10700	2135420..2135782	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAA6159_RS10705	2135779..2135949	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAA6159_RS10710	2136034..2137182	-	1149	WP_001281148.1	alanine racemase	-
SAA6159_RS10715	2137248..2137607	-	360	WP_000581196.1	holo-ACP synthase	-
SAA6159_RS10720	2137611..2138102	-	492	WP_001205916.1	PH domain-containing protein	-
SAA6159_RS10725	2138089..2139672	-	1584	WP_001294649.1	PH domain-containing protein	-
SAA6159_RS10730	2139665..2140144	-	480	WP_000118169.1	hypothetical protein	-
SAA6159_RS10735	2140353..2140913	-	561	WP_001092416.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T26613 WP_000621175.1 NC_017338:c2135782-2135420 [Staphylococcus aureus subsp. aureus JKD6159]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T26613 NC_017338:c2135782-2135420 [Staphylococcus aureus subsp. aureus JKD6159]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT26613 WP_000948331.1 NC_017338:c2135949-2135779 [Staphylococcus aureus subsp. aureus JKD6159]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT26613 NC_017338:c2135949-2135779 [Staphylococcus aureus subsp. aureus JKD6159]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1