Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 22858..23995 Replicon plasmid pEFM61-1
Accession NZ_CP113833
Organism Enterococcus faecalis strain M61

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag OZF29_RS13560 Protein ID WP_002332783.1
Coordinates 23132..23995 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag OZF29_RS13555 Protein ID WP_002326825.1
Coordinates 22858..23130 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OZF29_RS13515 (OZF29_13515) 17874..17957 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
OZF29_RS13520 (OZF29_13520) 18082..18819 + 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
OZF29_RS13525 (OZF29_13525) 18823..19617 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
OZF29_RS13530 (OZF29_13530) 20159..20242 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
OZF29_RS13535 (OZF29_13535) 20367..21104 + 738 WP_001038789.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
OZF29_RS13540 (OZF29_13540) 21274..21534 + 261 Protein_22 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
OZF29_RS13545 (OZF29_13545) 21637..22533 + 897 WP_002326827.1 ParA family protein -
OZF29_RS13550 (OZF29_13550) 22625..22840 + 216 WP_001835296.1 peptide-binding protein -
OZF29_RS13555 (OZF29_13555) 22858..23130 + 273 WP_002326825.1 antitoxin Antitoxin
OZF29_RS13560 (OZF29_13560) 23132..23995 + 864 WP_002332783.1 zeta toxin family protein Toxin
OZF29_RS13565 (OZF29_13565) 24435..24752 + 318 WP_002326830.1 hypothetical protein -
OZF29_RS13570 (OZF29_13570) 24952..25248 + 297 WP_002311824.1 hypothetical protein -
OZF29_RS13575 (OZF29_13575) 25255..27207 + 1953 WP_000163792.1 hypothetical protein -
OZF29_RS13580 (OZF29_13580) 27279..27776 - 498 WP_000868795.1 trimethoprim-resistant dihydrofolate reductase DfrG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cat / tet(L) / tet(M) / erm(B) / aph(3')-III / dfrG bsh 1..73413 73413
- flank IS/Tn erm(B) / aph(3')-III - 16688..21104 4416


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T266128 WP_002332783.1 NZ_CP113833:23132-23995 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT266128 WP_002326825.1 NZ_CP113833:22858-23130 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References