Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 49064..49635 Replicon plasmid pEFT30-1
Accession NZ_CP113829
Organism Enterococcus faecalis strain T30

Toxin (Protein)


Gene name mazF Uniprot ID A0A2Z2CGM4
Locus tag OZE10_RS00275 Protein ID WP_002362432.1
Coordinates 49064..49405 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag OZE10_RS00280 Protein ID WP_002362431.1
Coordinates 49405..49635 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OZE10_RS00255 (OZE10_00255) 44463..45746 - 1284 Protein_50 hypothetical protein -
OZE10_RS00265 (OZE10_00265) 47147..47749 - 603 WP_002362434.1 Fic family protein -
OZE10_RS00270 (OZE10_00270) 48014..48952 - 939 WP_002362433.1 hypothetical protein -
OZE10_RS00275 (OZE10_00275) 49064..49405 - 342 WP_002362432.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OZE10_RS00280 (OZE10_00280) 49405..49635 - 231 WP_002362431.1 hypothetical protein Antitoxin
OZE10_RS00285 (OZE10_00285) 49839..50459 + 621 WP_013438829.1 recombinase family protein -
OZE10_RS00290 (OZE10_00290) 50476..50760 + 285 WP_002394798.1 hypothetical protein -
OZE10_RS00295 (OZE10_00295) 50762..50995 + 234 WP_002394799.1 hypothetical protein -
OZE10_RS00300 (OZE10_00300) 51155..51409 - 255 WP_002394800.1 hypothetical protein -
OZE10_RS00305 (OZE10_00305) 51526..52194 - 669 WP_002394801.1 CPBP family intramembrane metalloprotease -
OZE10_RS00310 (OZE10_00310) 52230..52547 - 318 WP_002394802.1 heavy metal-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(L) / tet(M) / fexA / optrA / erm(A) / erm(B) - 1..65374 65374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13244.44 Da        Isoelectric Point: 8.0113

>T266079 WP_002362432.1 NZ_CP113829:c49405-49064 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT266079 WP_002362431.1 NZ_CP113829:c49635-49405 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z2CGM4

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References