Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 56569..57305 Replicon plasmid pBSKP1
Accession NZ_CP113790
Organism Klebsiella variicola subsp. tropica strain BSK_177V2

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag OUI59_RS27765 Protein ID WP_003026803.1
Coordinates 56823..57305 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag OUI59_RS27760 Protein ID WP_003026799.1
Coordinates 56569..56835 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OUI59_RS27740 (OUI59_27740) 51914..53731 + 1818 WP_000925242.1 multicopper oxidase PcoA -
OUI59_RS27745 (OUI59_27745) 53737..54627 + 891 WP_001378118.1 copper resistance outer membrane transporter PcoB -
OUI59_RS27750 (OUI59_27750) 54667..55050 + 384 WP_268657995.1 copper resistance system metallochaperone PcoC -
OUI59_RS27755 (OUI59_27755) 55072..56040 + 969 WP_072202995.1 IS5 family transposase -
OUI59_RS27760 (OUI59_27760) 56569..56835 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
OUI59_RS27765 (OUI59_27765) 56823..57305 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
OUI59_RS27770 (OUI59_27770) 57517..58863 + 1347 WP_077251107.1 ISNCY family transposase -
OUI59_RS27775 (OUI59_27775) 59023..59727 + 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -
OUI59_RS27780 (OUI59_27780) 60062..60319 - 258 WP_023292103.1 hypothetical protein -
OUI59_RS27785 (OUI59_27785) 60961..61476 + 516 Protein_66 CSS-motif domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..154584 154584
- inside IScluster/Tn - - 55072..58863 3791


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T266060 WP_003026803.1 NZ_CP113790:56823-57305 [Klebsiella variicola subsp. tropica]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT266060 WP_003026799.1 NZ_CP113790:56569-56835 [Klebsiella variicola subsp. tropica]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References