Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5526730..5527355 Replicon chromosome
Accession NZ_CP113789
Organism Klebsiella variicola subsp. tropica strain BSK_177V2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OUI59_RS27075 Protein ID WP_002882817.1
Coordinates 5526730..5527113 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OUI59_RS27080 Protein ID WP_004150355.1
Coordinates 5527113..5527355 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OUI59_RS27060 (OUI59_27060) 5524096..5524998 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OUI59_RS27065 (OUI59_27065) 5524995..5525630 + 636 WP_061156261.1 formate dehydrogenase cytochrome b556 subunit -
OUI59_RS27070 (OUI59_27070) 5525627..5526556 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OUI59_RS27075 (OUI59_27075) 5526730..5527113 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OUI59_RS27080 (OUI59_27080) 5527113..5527355 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OUI59_RS27085 (OUI59_27085) 5527560..5528477 + 918 WP_074387461.1 alpha/beta hydrolase -
OUI59_RS27090 (OUI59_27090) 5528492..5529433 - 942 WP_165784254.1 fatty acid biosynthesis protein FabY -
OUI59_RS27095 (OUI59_27095) 5529478..5529915 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
OUI59_RS27100 (OUI59_27100) 5529912..5530772 - 861 WP_074387464.1 virulence factor BrkB family protein -
OUI59_RS27105 (OUI59_27105) 5530766..5531365 - 600 WP_100632113.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T266058 WP_002882817.1 NZ_CP113789:c5527113-5526730 [Klebsiella variicola subsp. tropica]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT266058 WP_004150355.1 NZ_CP113789:c5527355-5527113 [Klebsiella variicola subsp. tropica]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References