Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3154455..3155045 Replicon chromosome
Accession NZ_CP113784
Organism Citrobacter freundii strain CFSAN077772

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag OW080_RS14780 Protein ID WP_003836692.1
Coordinates 3154713..3155045 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag OW080_RS14775 Protein ID WP_003836694.1
Coordinates 3154455..3154712 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OW080_RS14770 (3149942) 3149942..3153841 - 3900 WP_032936164.1 hypothetical protein -
OW080_RS14775 (3154455) 3154455..3154712 + 258 WP_003836694.1 hypothetical protein Antitoxin
OW080_RS14780 (3154713) 3154713..3155045 + 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OW080_RS14790 (3155520) 3155520..3156437 + 918 WP_003030855.1 nitrogen assimilation transcriptional regulator NAC -
OW080_RS14795 (3156539) 3156539..3157489 + 951 WP_003846751.1 HTH-type transcriptional regulator Cbl -
OW080_RS14805 (3157805) 3157805..3159292 - 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3135113..3166480 31367
- inside Genomic island - - 3135113..3167488 32375


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T266038 WP_003836692.1 NZ_CP113784:3154713-3155045 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT266038 WP_003836694.1 NZ_CP113784:3154455-3154712 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References