Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1355333..1355949 Replicon chromosome
Accession NZ_CP113784
Organism Citrobacter freundii strain CFSAN077772

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag OW080_RS06445 Protein ID WP_003028682.1
Coordinates 1355575..1355949 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag OW080_RS06440 Protein ID WP_043018956.1
Coordinates 1355333..1355575 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OW080_RS06415 (1351183) 1351183..1351782 + 600 WP_003028663.1 glucose-1-phosphatase -
OW080_RS06420 (1351776) 1351776..1352648 + 873 WP_269019838.1 virulence factor BrkB family protein -
OW080_RS06425 (1352645) 1352645..1353082 + 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
OW080_RS06430 (1353127) 1353127..1354068 + 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
OW080_RS06435 (1354219) 1354219..1355127 - 909 WP_003847901.1 alpha/beta hydrolase -
OW080_RS06440 (1355333) 1355333..1355575 + 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
OW080_RS06445 (1355575) 1355575..1355949 + 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
OW080_RS06450 (1355986) 1355986..1356915 - 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
OW080_RS06455 (1356912) 1356912..1357547 - 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
OW080_RS06460 (1357544) 1357544..1358446 - 903 WP_003847898.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T266033 WP_003028682.1 NZ_CP113784:1355575-1355949 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT266033 WP_043018956.1 NZ_CP113784:1355333-1355575 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References