Detailed information of TA system TA026597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2178885..2179399
• Replicon: chromosome
• Accession: NC_017337
• Organism: Staphylococcus aureus subsp. aureus ED133

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: SAOV_RS11185
• Protein ID: WP_000581796.1
• Coordinates: 2178885..2179094 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: SAOV_RS11190
• Protein ID: WP_000138932.1
• Coordinates: 2179106..2179399 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAOV_RS11165	2174390..2175748	-	1359	WP_000611455.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SAOV_RS11170	2175763..2176833	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
SAOV_RS11175	2177151..2178353	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
SAOV_RS11180	2178620..2178757	-	138	WP_000828354.1	hypothetical protein	-
SAOV_RS11185	2178885..2179094	-	210	WP_000581796.1	heavy-metal-associated domain-containing protein	Toxin
SAOV_RS11190	2179106..2179399	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SAOV_RS11195	2179565..2181049	+	1485	WP_000571548.1	cardiolipin synthase	-
SAOV_RS11200	2181077..2181724	+	648	WP_001187615.1	HD domain-containing protein	-
SAOV_RS11205	2182461..2183333	-	873	WP_000725799.1	membrane protein insertase YidC	-
SAOV_RS11210	2183419..2184060	-	642	WP_000483160.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7859.77 Da
• Isoelectric Point: 4.1008

>T26597 WP_000581796.1 NC_017337:c2179094-2178885 [Staphylococcus aureus subsp. aureus ED133]
MIHQNTIYTSGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T26597 NC_017337:c2179094-2178885 [Staphylococcus aureus subsp. aureus ED133]
ATGATACATCAAAATACGATTTACACATCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT26597 WP_000138932.1 NC_017337:c2179399-2179106 [Staphylococcus aureus subsp. aureus ED133]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT26597 NC_017337:c2179399-2179106 [Staphylococcus aureus subsp. aureus ED133]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: W8UW13