Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 395254..395891 Replicon chromosome
Accession NZ_CP113515
Organism Bacillus sp. KICET-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OVA33_RS02060 Protein ID WP_003156187.1
Coordinates 395254..395604 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag OVA33_RS02065 Protein ID WP_003156188.1
Coordinates 395610..395891 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OVA33_RS02020 (390294) 390294..390896 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
OVA33_RS02025 (390896) 390896..391684 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
OVA33_RS02030 (391650) 391650..392132 - 483 WP_003156173.1 anti-sigma B factor RsbW -
OVA33_RS02035 (392129) 392129..392458 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
OVA33_RS02040 (392522) 392522..393529 - 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
OVA33_RS02045 (393541) 393541..393942 - 402 WP_017419223.1 anti-sigma regulatory factor -
OVA33_RS02050 (393945) 393945..394310 - 366 WP_007609584.1 RsbT antagonist protein RsbS -
OVA33_RS02055 (394315) 394315..395136 - 822 WP_003156182.1 STAS domain-containing protein -
OVA33_RS02060 (395254) 395254..395604 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OVA33_RS02065 (395610) 395610..395891 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OVA33_RS02070 (396011) 396011..397180 - 1170 WP_065180972.1 alanine racemase -
OVA33_RS02075 (397297) 397297..398304 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
OVA33_RS02080 (398469) 398469..398834 - 366 WP_007609580.1 holo-ACP synthase -
OVA33_RS02085 (398927) 398927..399526 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T265858 WP_003156187.1 NZ_CP113515:c395604-395254 [Bacillus sp. KICET-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT265858 WP_003156188.1 NZ_CP113515:c395891-395610 [Bacillus sp. KICET-1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References