Detailed information of TA system TA026583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2209194..2209708
• Replicon: chromosome
• Accession: NC_017333
• Organism: Staphylococcus aureus subsp. aureus ST398

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: SAPIG_RS11155
• Protein ID: WP_000581792.1
• Coordinates: 2209194..2209403 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: SAPIG_RS11160
• Protein ID: WP_000138932.1
• Coordinates: 2209415..2209708 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAPIG_RS11135	2204705..2206060	-	1356	WP_000611457.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SAPIG_RS11140	2206075..2207145	-	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
SAPIG_RS11145	2207463..2208665	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
SAPIG_RS11150	2208929..2209066	-	138	WP_000828354.1	hypothetical protein	-
SAPIG_RS11155	2209194..2209403	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
SAPIG_RS11160	2209415..2209708	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SAPIG_RS11165	2209874..2211358	+	1485	WP_000571550.1	cardiolipin synthase	-
SAPIG_RS11170	2211386..2212033	+	648	WP_001187614.1	HD domain-containing protein	-
SAPIG_RS11175	2212313..2212633	+	321	WP_001825748.1	hypothetical protein	-
SAPIG_RS11180	2212630..2213945	-	1316	Protein_2117	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T26583 WP_000581792.1 NC_017333:c2209403-2209194 [Staphylococcus aureus subsp. aureus ST398]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T26583 NC_017333:c2209403-2209194 [Staphylococcus aureus subsp. aureus ST398]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT26583 WP_000138932.1 NC_017333:c2209708-2209415 [Staphylococcus aureus subsp. aureus ST398]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT26583 NC_017333:c2209708-2209415 [Staphylococcus aureus subsp. aureus ST398]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13